The colourful motion graphics will illuminate the iconic building's sails to help launch the Vivid Sydney light festival.
"Each creature represents a different emotion," says Ash Bolland, a music video and TV advert director who conceived the display.
"It's based on the idea of turning the opera house into a living, breathing entity."
Bolland's mesmerising spectacle, Audio Creatures, will be set to electronic music by a Brazilian producer, Amon Tobin.
As with previous years, crowds are expected to pack the harbour-side setting and other locations for the popular winter festival.